Sukara, Endang and Kumagai, Hidehiko and Yamamoto, Kenji (1998) STUDY ON AMYLOGLUCOSIDASE OF A NEWLY ISOLATED Saccharomycopsis sp. TJ-1 FROM THE INDONESIAN FERMENTED FOOD (TAPE). Annales Bogorienses, n.s., 5 (2).
![[img]](http://repository.unas.ac.id/style/images/fileicons/text.png) |
Text
Endang Sukara.pdf Download (548kB) |
Abstract
SDS-PAGE analysis showed that fermentation broth of Saccharomycopsis sp TJ-1 contained only one distinct band of protein at approximately 67 kd and one smear band at around 19 kd. Gel filtration studies showed that there were 3 peaks at A280, but only the last two peaks showing glucoamylase (AMG) activity. Observation using native gradient PAGE on an active fractions of gel filtration showed that there was only one band of protein and showed a flourescent spot under UV light when the gel was exposed to 4-methyl-belliferyl aa-D glucoside in 10 mM phosphate citrate buffer. This lead to a preliminary conclusion, that Saccharomycopsis sp. TJ-1 contains only 1 type of AMG. The N-terminal amino acid sequence of purified product is AYP SFEAYSNYKV DXTDLET (Ala-Tyr-Pro-Ser-Phe-Glu-Ala-iyr-Ser-Asn-Tyr-Lys-Val-Asp-X-Thr-Asp-Asp-Leu-Glu-Thr) corresponse to the amino acid sequence of AMG gene GLA1 from E. fibuligera KZ at the sequence position between 28 and 47. .
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Artikel > Biologi |
| Depositing User: | BPSI Unas |
| Date Deposited: | 10 Aug 2019 08:56 |
| Last Modified: | 10 Aug 2019 08:56 |
| URI: | http://repository.unas.ac.id/id/eprint/379 |
Actions (login required)
 |
View Item |
